Cell Meter RX780固定化细胞活性染料

       

产品参数

Ex (nm)	629	Em (nm)	767
分子量	1176.38	溶剂	DMSO
存储条件	在零下15度以下保存, 避免光照

   简要概述

产品基本信息

货号：22536

产品名称：Cell Meter RX780固定化细胞活性染料

规格：200 Tests

储存条件：保存在冰箱-15℃干燥

保质期：12个月

产品物理化学光谱特性

分子量：N/A

激发波长(nm)：629

发射波长(nm)：767

  产品介绍

从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter RX780固定化细胞活性染料是一类细胞不可渗透的荧光活性染料，它们经过优化以匹配常见流式细胞仪的主要激发激光，例如350、405、488、633和647 nm。这些染料不渗入活细胞，但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter RX780固定化细胞活性染料发生反应，因此比具有完整膜的活细胞的染色更亮，因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时，需要考虑一些因素，例如，每种染料的滴定，以确保活细胞几乎没有染色。Cell Meter RX780固定化细胞活性染料经过优化，可以在633/647 nm的红色激光激发下以780 nm的波长发射。与其他商业上类似的活性染料相比，这种固定化细胞活性染料更加耐用、稳定。百萤生物是AAT Bioquest的中国代理商，为您提供优质的Cell Meter RX780固定化细胞活性染料。 

图示

图1.通过Cell Meter 固定化细胞活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter RX780（Cat＃22536）染色，然后固定在3.7％甲醛中，并通过流式细胞仪进行分析。通过APC-Cy7通道可以很容易地将死细胞群体（蓝峰）与活细胞群体（红峰）区分开，在固定之前和之后可获得几乎相同的结果

参考文献

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The miR-3940-5p inhibits cell proliferation of gingival mesenchymal stem cells.Authors: Han, Xiao and Yang, Haoqing and Cao, Yangyang and Ge, Lihua and Han, Nannan and Zhang, Chen and Fan, Zhipeng and Yao, RuiJournal: Oral diseases (2019): 1363-1373

 

Toll-Like Receptor 4 (TLR4)/Cyclooxygenase-2 (COX-2) Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion by NF-κB Activation.Authors: Wang, Wei and Wang, JiyeJournal: Medical science monitor : international medical journal of experimental and clinical research (2018): 5588-5597

 

Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing.Authors: Nagarkatti, Mitzi and Rieder, Sadiye Amcaoglu and Nagarkatti, Prakash SJournal: Methods in molecular biology (Clifton, N.J.) (2018): 209-230

 

Quantitative analysis of cell proliferation by a dye dilution assay: Application to cell lines and cocultures.Authors: Chung, Soobin and Kim, Seol-Hee and Seo, Yuri and Kim, Sook-Kyung and Lee, Ji YounJournal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2017): 704-712

 

[Myeloid-derived suppressor cells promoted autologous B cell proliferation in rheumatoid arthritis].Authors: Chen, W and Hu, F L and Liu, H J and Xu, L L and Li, Y N and Li, Z GJournal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (2017): 819-823

 

Gag-Specific CD4 T Cell Proliferation, Plasmacytoid Dendritic Cells, and Ethnicity in Perinatally HIV-1-Infected Youths: The ANRS-EP38-IMMIP Study.Authors: Scott-Algara, Daniel and Warszawski, Josiane and Chenadec, Jérôme Le and Didier, Céline and Montange, Thomas and Viard, Jean-Paul and Dollfus, Catherine and Avettand-Fenoel, Véronique and Rouzioux, Christine and Blanche, Stéphane and Buseyne, FlorenceJournal: AIDS research and human retroviruses (2017): 21-28

 

Hypoxia inhibits mesenchymal stem cell proliferation through HIF1α-dependent regulation of P27.Authors: Kumar, Sanjay and Vaidya, MeenalJournal: Molecular and cellular biochemistry (2016): 29-38

 

Vanadium pentoxide prevents NK-92MI cell proliferation and IFNγ secretion through sustained JAK3 phosphorylation.Authors: Gallardo-Vera, Francisco and Diaz, Daniel and Tapia-Rodriguez, Miguel and Fortoul van der Goes, Teresa and Masso, Felipe and Rendon-Huerta, Erika and Montaño, Luis FJournal: Journal of immunotoxicology (2016): 27-37

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