AAT BioquestCytoCalcein Violet 660，钠盐 *在 405 nm 处激发* 货号21903

产品参数

Ex (nm)	-	Em (nm)	-
分子量	N/A	溶剂	DMSO
存储条件	在零下15度以下保存, 避免光照

产品概述

产品基本信息

产品名称：CytoCalcein™ Violet 660，钠盐 *在 405 nm 处激发*

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：662.63

外观：固体

溶剂：DMSO

激发波长(nm)：N/A

发射波长(nm)：N/A

 

选择指南

Dye		Calcein Blue	CytoCalcein™ Violet 450	Calcein UltraBlue™	CytoCalcein™ Violet 500	Calcein UltraGreen™	Calcein	Calcein Orange™	Calcein Red™	Calcein Deep Red™
原理		在活细胞中，非荧光钙黄绿素 AM 在细胞内酯酶的作用下 AM 酯水解后转化为荧光钙黄绿素产物。
荧光效果		活细胞具有高度荧光，很容易与无荧光的死细胞区分开。
Laser(nm)		UV (350)	Violet (405)	UV (350)	Violet (405)	Blue (488)	Blue (488)	Green (532)	Yellow (561-568)	Red (633-647)
Ex/Em(nm)		354/441	406/445	359/458	420/505	492/514	501/521	531/545	562/576	643/663
是否复用		是	是	是	是	是	是	是	是	是
染料相容性		与绿色和红色荧光蛋白、指示剂和其他探针兼容	与绿色和红色荧光蛋白、指示剂和其他探针兼容	与绿色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和绿色荧光蛋白、指示剂和其他探针兼容	与蓝色和绿色荧光蛋白、指示剂和其他探针兼容
样品类型		活细胞
适用仪器		荧光显微镜、荧光酶标仪、流式细胞仪
货号		22007	22012	21908	22013	21905	22003	22009	21900	22011

 

产品介绍

CytoCalcein™ Violet 660 是 CytoCalcein™ Violet 660 AM 在活细胞中的酯酶裂解产物。 CytoCalcein™ Violet 660 不具有细胞渗透性。它是 CytoCalcein™ Violet 660 AM 的参考产品，设计用于以与 Calcein, AM 相同的方式标记活细胞。 CytoCalcein™ Violet 660 可以被 405 nm 的紫激光很好地激发，目前大多数流式细胞仪都配备了这种激光。它也能被荧光显微镜的激发源充分激发。这种与典型的现有 FACS 荧光团的特殊光谱分离为流式细胞仪或荧光显微镜的多重实验提供了额外的选择。 CytoCalcein™ Violet 660 专为多重流式细胞术和荧光成像应用而开发。它为活细胞的流式细胞术分析提供了一种新的独特颜色。百萤生物是AAT Bioquest的中国代理商，为您提供优质的CytoCalcein™ Violet 660，钠盐。 

 

溶解方案(溶剂以说明书为准)

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	150.914 µL	754.569 µL	1.509 mL	7.546 mL	15.091 mL
5 mM	30.183 µL	150.914 µL	301.828 µL	1.509 mL	3.018 mL
10 mM	15.091 µL	75.457 µL	150.914 µL	754.569 µL	1.509 mL

 

参考文献

A Ratiometric Probe Based on Carbon Dots and Calcein & Eu3+ for the Fluorescent Detection of Sodium Tripolyphosphate.Authors: Zheng, Yabin and Li, Chen and Li, Quan and Zhang, Taoyi and Chen, Jing and Ji, Wenxi and Wei, YunJournal: Journal of fluorescence (2023): 965-972

 

Dual-emission fluorescence detection of histidine using carbon dots and calcein/Ni2+ complexes.Authors: Liu, Jinshui and Song, Bo and Fu, TingJournal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 121951

 

Construction of N-CDs and Calcein-Based Ratiometric Fluorescent Sensor for Rapid Detection of Arginine and Acetaminophen.Authors: Qi, Haiyan and Li, Qiuying and Jing, Jing and Jing, Tao and Liu, Chuntong and Qiu, Lixin and Sami, Rokayya and Helal, Mahmoud and Ismail, Khadiga Ahmed and Aljahani, Amani HJournal: Nanomaterials (Basel, Switzerland) (2022)

 

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters.Authors: Hausig-Punke, Franziska and Richter, Friederike and Hoernke, Maria and Brendel, Johannes C and Traeger, AnjaJournal: Macromolecular bioscience (2022): e2200167

 

Sensitive ratiometric fluorescence probe based on chitosan carbon dots and calcein for Alkaline phosphatase detection and bioimaging in cancer cells.Authors: Mu, Xiaowei and Jiang, Xue and Zhang, Yu and Liu, Xin and Zhang, Siqi and Wang, Wei and Huang, Yibing and Ma, Pinyi and Song, DaqianJournal: Analytica chimica acta (2021): 339163

 

Calcein Binding to Assess Mineralization in Hydrogel Microspheres.Authors: White, Kristopher and Chalaby, Rabab and Lowe, Gina and Berlin, Jacob and Glackin, Carlotta and Olabisi, RonkeJournal: Polymers (2021)

 

Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay.Authors: Lorenzo-Herrero, Seila and Sordo-Bahamonde, Christian and González, Segundo and López-Soto, AlejandroJournal: Methods in enzymology (2020): 483-495

 

Calcein Fluorescence Quenching to Measure Plasma Membrane Water Flux in Live Mammalian Cells.Authors: Kitchen, Philip and Salman, Mootaz M and Abir-Awan, Mohammed and Al-Jubair, Tamim and Törnroth-Horsefield, Susanna and Conner, Alex C and Bill, Roslyn MJournal: STAR protocols (2020): 100157

 

Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein.Authors: Dutta, Sayan and Watson, Ben G and Mattoo, Seema and Rochet, Jean-ChristopheJournal: Bio-protocol (2020)

 

The Relationship Between Water Transmission Rate and Defects on the Film Based on the Defect Analysis Using Fluorescent Calcein Probe.Authors: Kim, Sun Min and Kim, Hyun Chul and Kim, Chang-Yeoul and Yoon, Daeho and Koo, EunhaeJournal: Journal of nanoscience and nanotechnology (2020): 5469-5472

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