AAT Bioquest CytoCalcein Violet 660，AM 货号21904

产品参数

Ex (nm)	-	Em (nm)	-
分子量	N/A	溶剂	DMSO
存储条件	在零下15度以下保存, 避免光照

产品概述

产品基本信息

产品名称：CytoCalcein™ Violet 660，AM *在 405 nm 处激发*

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：812.87

外观：固体

溶剂：DMSO

激发波长(nm)：N/A

发射波长(nm)：N/A

 

选择指南

Dye		Calcein Blue	CytoCalcein™ Violet 450	Calcein UltraBlue™	CytoCalcein™ Violet 500	Calcein UltraGreen™	Calcein	Calcein Orange™	Calcein Red™	Calcein Deep Red™
原理		在活细胞中，非荧光钙黄绿素 AM 在细胞内酯酶的作用下 AM 酯水解后转化为荧光钙黄绿素产物。
荧光效果		活细胞具有高度荧光，很容易与无荧光的死细胞区分开。
Laser(nm)		UV (350)	Violet (405)	UV (350)	Violet (405)	Blue (488)	Blue (488)	Green (532)	Yellow (561-568)	Red (633-647)
Ex/Em(nm)		354/441	406/445	359/458	420/505	492/514	501/521	531/545	562/576	643/663
是否复用		是	是	是	是	是	是	是	是	是
染料相容性		与绿色和红色荧光蛋白、指示剂和其他探针兼容	与绿色和红色荧光蛋白、指示剂和其他探针兼容	与绿色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和红色荧光蛋白、指示剂和其他探针兼容	与蓝色和绿色荧光蛋白、指示剂和其他探针兼容	与蓝色和绿色荧光蛋白、指示剂和其他探针兼容
样品类型		活细胞
适用仪器		荧光显微镜、荧光酶标仪、流式细胞仪
货号		22007	22012	21908	22013	21905	22003	22009	21900	22011

 

产品介绍

CytoCalcein Violet 660 AM 酯设计用于以与 Calcein, AM 相同的方式标记活细胞。 405 nm 的紫激光可以很好地激发它，目前大多数流式细胞仪都配备了这种激光。它也能被荧光显微镜的激发源充分激发。 CytoCalcein™ Violet 660 进入活细胞后会水解成带有多个负电荷的荧光染料，这使得染料能够很好地保留在活细胞中。它在已知的活细胞染色剂中具有zui大的斯托克斯位移。这种与典型的现有 FACS 荧光团的特殊光谱分离为流式细胞仪或荧光显微镜的多重实验提供了额外的选择。 CytoCalcein™ Violet 660 专为多重流式细胞术和荧光成像应用而开发。它为活细胞的流式细胞术分析提供了一种新的独特颜色。百萤生物是AAT Bioquest的中国代理商，为您提供优质的CytoCalcein™ Violet 660，AM。 

 

溶解方案(溶剂以说明书为准)

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	123.021 µL	615.105 µL	1.23 mL	6.151 mL	12.302 mL
5 mM	24.604 µL	123.021 µL	246.042 µL	1.23 mL	2.46 mL
10 mM	12.302 µL	61.51 µL	123.021 µL	615.105 µL	1.23 mL

 

参考文献

Neuroprotective Effects of Ethanol Extract of Polyscias fruticosa (EEPF) against Glutamate-Mediated Neuronal Toxicity in HT22 Cells.Authors: Selvaraj, Baskar and Le, Tam Thi and Kim, Dae Won and Jung, Bo Hyun and Yoo, Ki-Yeon and Ahn, Hong Ryul and Thuong, Phuong Thien and Tran, Thi Thu Thuy and Pae, Ae Nim and Jung, Sang Hoon and Lee, Jae WookJournal: International journal of molecular sciences (2023)

 

Drug-free and non-crosslinked chitosan/hyaluronic acid hybrid hydrogel for synergistic healing of infected diabetic wounds.Authors: Lin, Yao and Xu, Jingchen and Dong, Yuanhao and Wang, Yue and Yu, Chenhao and Li, Yuanhong and Zhang, Chaoliang and Chen, Qianming and Chen, Song and Peng, QiangJournal: Carbohydrate polymers (2023): 120962

 

In Situ Activation of the Receptor Aggregation for Cell Apoptosis by an AI-Au Intelligent Nanomachine via Tumor Extracellular Acidity.Authors: Zhang, Yue and Cheng, Feng and Liang, Rui and Shuai, Xin Jia and Nie, Kun Han and Li, Jing and Zeng, Qiao Ling and Huang, Cheng Zhi and Li, Chun MeiJournal: ACS applied materials & interfaces (2023): 32262-32271

 

Long non-coding RNA MRPL23-AS1 suppresses anoikis in salivary adenoid cystic carcinoma in vitro.Authors: Li, Yin-Ran and Fu, Min and Song, Ye-Qing and Li, Sheng-Lin and Ge, Xi-YuanJournal: Oral diseases (2023): 1588-1601

 

Synthesis of Pt nanoparticles with gelatin-assisted green route to improve sensitization of cancer cells to X-Ray irradiation.Authors: Charmi, Jalil and Seidi, Farzad and Amereh, Mahdi and Ghaffarlou, Mohammadreza and Salehiabar, Marziyeh and Yousefnejad, Shima and Barsbay, Murat and Sharafi, Ali and Javani, Siamack and Nosrati, HamedJournal: International journal of pharmaceutics (2023): 123148

 

HSP90-Dependent Upregulation of EZH2 Promotes Hypoxia/Reoxygenation-Induced Pyroptosis by Inhibiting miR-22 in Endothelial Cells.Authors: Deng, Paihe and Hu, HuiminJournal: Journal of inflammation research (2023): 2615-2630

 

Measuring chondrocyte viability of articular cartilage based on label-free two-photon microscopy and deep learning image analysis.Authors: Fan, Hongming and Xu, Pei and Chen, Xun and Li, Yang and Hsu, Jennifer and Le, Michael and Zhang, Zhao and Ye, Emily and Gao, Bruce and Ye, TongJournal: bioRxiv : the preprint server for biology (2023)

 

Cellular responses to 8-methyl nonanoic acid, a degradation by-product of dihydrocapsaicin, in 3T3-L1 adipocytes.Authors: Wichai, Uthai and Keawsomnuk, Ploychanok and Thongin, Saowarose and Mukthung, Chaiyot and Boonthip, Chatchai and Pittayakhajonwut, Pattama and Ketsawatsomkron, Pimonrat and Bunyapraphatsara, Nuntavan and Muta, KenjiroJournal: BMC complementary medicine and therapies (2023): 18

 

Red clover (Trifolium pratense L.) extract inhibits ferroptotic cell death by modulating cellular iron homeostasis.Authors: Won, Jun Pil and Kim, Eunsu and Hur, Jinwoo and Lee, Hyuk Gyoon and Lee, Won Jin and Seo, Han GeukJournal: Journal of ethnopharmacology (2023): 116267

 

Silver sulfide coated alginate radioenhancer for enhanced X-ray radiation therapy of breast cancer.Authors: Mousazadeh, Navid and Seidi, Farzad and Ghaffarlou, Mohammadreza and Rashidzadeh, Hamid and Hosseinmirzaei, Ali and Mozafari, Faezeh and Danafar, Hossein and Nosrati, HamedJournal: International journal of biological macromolecules (2023): 123636

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